No. There is an initial “Where is my family going?” when she comes to us, but in every situation, the dog is settled and comfortable and doing very well within an hour or two. Remember they will already “know” us from the times we have taken them for testing or when we have “dog sat” for you. We try very hard to give them so much attention and love the first couple days that it is a pleasant and enjoyable experience for them. This is also important as everything the mother feels causes things to happen inside her body that can affect the babies. The less stress and the more relaxed she is, the better it is for babies. So, it is very important that the guardian home not make the transition difficult for the dog. If they act upset or nervous or sad about leaving her, she will feel that even more greatly and we need to make sure that doesn’t happen. Bringing her and hanging out with her for an hour or so and just pretending like it’s any other visit you’d make is very important. If we can have the family sneak out so the dog isn’t even aware they’ve left, that is usually best too. She rarely acknowledges for more than a couple of minutes that anything has happened.